VALIDATION IMAGES
Tissue: Human stomach Section type: Formalin-fixed & Paraffin-embedded
section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:1000 Primary Ab incubation condition: 1 hour at room
temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP
(Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60637R
Tissue: Human gastric carcinoma Section type: Formalin-fixed & Paraffin-embedded
section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab
dilution: 1:1000 Primary Ab incubation condition: 1 hour at room
temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP
(Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60637R
Tissue: Mouse stomach Section type: Formalin-fixed & Paraffin-embedded
section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab
dilution: 1:1000 Primary Ab incubation condition: 1 hour at room
temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP
(Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60637R
Tissue: Rat stomach Section type: Formalin-fixed & Paraffin-embedded
section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab
dilution: 1:1000 Primary Ab incubation condition: 1 hour at room
temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP
(Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60637R
Paraformaldehyde-fixed, paraffin embedded (Human stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Claudin 18) Monoclonal Antibody, Unconjugated (bsm-60637R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Claudin 18 ) Monoclonal Antibody, Unconjugated (bsm-60637R ) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.