bsm-60590R [Primary Antibody]
AATF monoclonal Antibody
www.biossusa.com
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DATASHEET

Host: Rabbit

Target Protein: AATF

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 26574

Swiss Prot: Q9NY61

Source: KLH conjugated synthetic peptide derived from human AATF

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

The protein encoded by this gene was identified on the basis of its interaction with MAP3K12/DLK, a protein kinase known to be involved in the induction of cell apoptosis. This gene product contains a leucine zipper, which is a characteristic motif of transcription factors, and was shown to exhibit strong transactivation activity when fused to Gal4 DNA binding domain. Overexpression of this gene interfered with MAP3K12 induced apoptosis. [provided by RefSeq, Jul 2008]

Size: 100ul

Concentration: 1mg/ml

Applications: WB=1:500-2000
ICC=1:50
IF=1:50-100

Predicted Molecular Weight: 63


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:1000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: 293T, 2: HeLa, 3: HepG2 Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 63 kDa Observed MW: 80 kDa