bsm-60567M
[Primary Antibody]
FAF2 Monoclonal Antibody
DATASHEET
Host: Mouse
Target Protein: FAF2
Clonality: Monoclonal
Isotype: IgG2B/Kappa
Swiss Prot: Q96CS3
Purification: Protein G.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: 1mg/ml
Applications:
WB(WB=1:500-1:1000)
IHC-P(IHC-P=1:50-1:100)
IHC-F(ICC/IF=1:50)
IF()
ICC/IF(IF=0)
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: room temperature 2h Secondary Ab: Goat Anti-Mouse IgG H&L (HRP) Lysate: 1: 293, 2: HepG2, 3: Mouse lung, 4: Rat liver Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 51 kDa Observed MW: 55 kDa
Tissue: Human breast cancer Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: SP Kit(Mouse)(sp-0024) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60567M