bsm-60489R
[Primary Antibody]
ALDOA Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: ALDOA
Clonality: Monoclonal
Isotype: IgG/Kappa
Swiss Prot: P04075
Purification: Protein G.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: 1mg/ml
Applications:
WB(1:300-5000)
IHC-P(1:200-400)
ICC(1:100-500)
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Tianming Lu. et al. Inhibition of ALDOA and ENO1-mediated Glycolysis of Oridonin as a Novel Anti-tumor Strategy of Non-small Cell Lung Cancer. Advanced Therapeutics. 2024 Apr;:2400027Read more>>
VALIDATION IMAGES
Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: MCF7, 2: HepG2, 3: Mouse kidney, 4: Rat brain Protein loading quantity: 20 μg Exposure time: 1 s Predicted MW: 39 kDa Observed MW: 35 kDa
Tissue: Human colon cancer Section type: Formalin-fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: SP Kit(Mouse)(sp-0024) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60489R