bsm-60449M [Primary Antibody]
14-3-3 sigma Recombinant Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: 14-3-3 sigma

Clonality: Recombinant

Isotype: IgG1/Kappa

Entrez Gene: 2810

Swiss Prot: P31947

Source: KLH conjugated synthetic peptide derived from human 14-3-3 sigma

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background:

This gene encodes a cell cycle checkpoint protein. The encoded protein binds to translation and initiation factors and functions as a regulator of mitotic translation. In response to DNA damage this protein plays a role in preventing DNA errors during mitosis. [provided by RefSeq, Aug 2017]

Size: 100ul

Concentration: 1mg/ml

Applications: WB=1:500-2000

Predicted Molecular Weight: 28


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:1000 Primary ab incubation condition: room temperature 2h Secondary ab: Goat Anti-Mouse IgG H&L (HRP) Lysate: HeLa, MCF-7 Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 28 kDa Observed MW: 28 kDa