bsm-60433R
[Primary Antibody]
CLDN1 Recombinant Antibody
DATASHEET
Host: Rabbit
Target Protein: CLDN1
Clonality: Recombinant
Isotype: IgG
Swiss Prot: O95832
Purification: Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: Lot dependent
Cross Reactive Species:
Human
Mouse
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Lechen Wang. et al. pH Sensitive Quercetin Nanoparticles Ameliorate DSS-Induced Colitis in Mice by Colon-Specific Delivery. MOL NUTR FOOD RES. 2023 Nov;:2300051Read more>>
- Li Lei. et al. Long-term Cu exposure alters CYP450s activity and induces jejunum injury and apoptosis in broilers. BIOMETALS. 2023 Nov;:1-12Read more>>
- Xinyun Qin. et al. Regulation of the intestinal flora using polysaccharides from Callicarpa nudiflora Hook to alleviate ulcerative colitis and the molecular mechanisms involved. INT J BIOL MACROMOL. 2024 Feb;258:128887Read more>>
- Wenjing Zhao. et al. Inhibitory Effects of Soluble Dietary Fiber from Foxtail Millet on Colorectal Cancer by the Restoration of Gut Microbiota. J AGR FOOD CHEM. 2024;72(21):1213012145Read more>>
VALIDATION IMAGES
Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: HepG2, 2: A431, 3: Mouse liver Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 22 kDa Observed MW: 18 kDa
25 ug total protein per lane of various lysates (see on figure) probed with CLDN1 monoclonal antibody, unconjugated (bsm-60433R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.