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Host: Rabbit
Target Protein: TSHB
Clonality: Recombinant
Isotype: IgG
Swiss Prot: P01222
Purification: Protein A.
Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage: Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: 1mg/ml
Applications:
WB(WB=1:500-1:2000)
IHC-P(IHC-P=1:100-500)
IHC-F(ICC/IF=1:50)
IF(Flow-Cyt=1:50-1:100)
Flow-Cyt(1ug/Test)
ICC/IF(IF=0)
Cross Reactive Species:
Human
Rat
For research use only. Not intended for diagnostic or therapeutic use.
Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: Rat pituitary Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 16 kDa Observed MW: 16 kDa
Tissue: Rat pituitary Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:500 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Rabbit) (sp-0023) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60432R