DATASHEET
Host:
Rabbit
Target Protein:
alpha Actinin 4
Clonality:
Monoclonal
Isotype:
IgG
Entrez Gene:
81
Swiss Prot:
O43707
Source:
KLH conjugated synthetic peptide derived from human alpha Actinin 4
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis. [provided by RefSeq, Jul 2008].
PRODUCT SPECIFIC PUBLICATIONS
- Qianru Chi. et al. In vitro and in silico analyses reveal the toxicity of metolachlor to grass carp hepatocytes and the antagonism of melatonin. PESTIC BIOCHEM PHYS. 2024 Apr;:10593Read more>>
VALIDATION IMAGES
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:5000
Primary ab incubation condition: 4°C overnight
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: 1:HeLa, 2:HEK-293, 3:4T1, 4:Raw264.7
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 100 kDa
Observed MW: 110 kDa
Tissue: Human colon Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Rabbit) (sp-0023) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60423R
Lane 1: Mouse Skin tissue lysates; Lane 2: Mouse Uterus tissue lysates; Lane 3: Human HeLa cell lysates; Lane 4: Human Jurkat cell lysates; Lane 5: Human U251 cell lysates probed with alpha Actinin 4 Monoclonal Antibody, Unconjugated (bsm-60423R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.