bsm-60374M
[Primary Antibody]
14-3-3 Monoclonal Antibody
DATASHEET
Host: Mouse
Target Protein: 3/3/14
Clonality: Monoclonal
Isotype: IgG1/Kappa
Swiss Prot: P27348
Purification: Protein G.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: 1mg/ml
Applications:
WB(1:300-5000)
ICC(1:100-500)
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 4°C overnight Secondary Ab: Goat Anti-Mouse IgG H&L (HRP) Lysate: 1: HeLa, 2: A549, 3: Neuro-2a, 4: PC-12 Protein loading quantity: 20 μg Exposure time: 10 s Predicted MW: 28 kDa Observed MW: 29 kDa