bsm-60354M
[Primary Antibody]
DBR1 Monoclonal Antibody
DATASHEET
Host: Mouse
Target Protein: DBR1
Clonality: Monoclonal
Isotype: IgG2b/Kappa
Swiss Prot: Q9UK59
Purification: Protein G.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: 1mg/ml
Applications:
WB(1:300-5000)
IHC-P(1:200-400)
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 4°C overnight Lysate: 1: Rat heart, 2: Mouse heart, 3: HeLa, 4: A431 Protein loading quantity: 20 μg Exposure time: 30 s Predicted MW: 75 kDa Observed MW: 75 kDa
Tissue: Mouse brain Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for bsm-60354M
Tissue: rat brain Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for bsm-60354M