bsm-60280R [Primary Antibody]
MAP4K4 monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
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DATASHEET

Host: Rabbit

Target Protein: MAP4K4

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 9448

Swiss Prot: O95819

Source: KLH conjugated synthetic peptide derived from human MAP4K4

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Size: 100ul

Concentration: 1mg/ml

Applications: IHC-P=1:200-500
ICC=1:50
IF=1:50-100

Predicted Molecular Weight: 142


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Tissue:Human prostate Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:50 Primary ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for bsm-60280R