bsm-60237R
[Primary Antibody]
CD43 Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: CD43
Clonality: Monoclonal
Isotype: IgG
Swiss Prot: P16150
Purification: Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: 1mg/ml
Applications:
WB(1:300-5000)
FCM(1:20-100)
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:5000 Primary ab incubation condition: 2 hours at room temperature Lysate: K562, THP-1 Protein loading quantity: 20 μg Exposure time: 30 s Predicted MW: 40 kDa Observed MW: 100-150 kDa
Lane 1: Human U937 cell lysates; Lane 2: Human K562 cell lysates; Lane 3: Human Jurkat cell lysates probed with CD43 Monoclonal Antibody, Unconjugated (bsm-60237R) at 1:10000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.