Host: Rabbit
Target Protein: CD22
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 933
Swiss Prot: P20273
Source: KLH conjugated synthetic peptide derived from human CD22
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1mg/ml
Applications:
WB=1:500-2000
IHC-P=1:400-800
ICC=1:100
IF=1:100-500
Predicted Molecular Weight: 91
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:1000 Primary ab incubation condition: 2 hours at room temperature Lysate: Daudi Protein loading quantity: 20 μg Exposure time: 15 s Predicted MW: 95 kDa Observed MW: 130 kDa
Tissue: Human spleen Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for bsm-60233R
Tissue: Human tonsil Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for bsm-60233R
Lane 1: Human Raji cell lysates; Lane 2: Human Raji cell lysates probed with CD22 Monoclonal Antibody, Unconjugated (bsm-60233R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.