bsm-60066M
[Primary Antibody]
Acetyl-Histone H4 (Lys5/8/12/16) Monoclonal Antibody
DATASHEET
Host: Mouse
Target Protein: Acetyl-Histone H4 (Lys5/8/12/16)
Clonality: Monoclonal
Isotype: IgG
Swiss Prot: P68431
Purification: Protein G.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: 1mg/ml
Applications:
WB(1:300-5000)
IHC-P(1:200-400)
ICC(1:100-500)
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Mouse IgG H&L (HRP) Lysate: (-) HeLa, (+) HeLa+Trichostatin A (2μM, 4hr) Protein loading quantity: 20 μg Exposure time: 30 s Predicted MW: 11 kDa Observed MW: 11 kDa
Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Mouse IgG H&L (HRP) Lysate: HeLa, N2a, BRL Protein loading quantity: 20 μg Exposure time: 30 s Predicted MW: 11 kDa Observed MW: 11 kDa
Tissue: Human neuroblastoma Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:500 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Mouse)(sp-0024) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60066M