bsm-60013M [Primary Antibody]
ROCK2 monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
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DATASHEET

Host: Mouse

Target Protein: ROCK2

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 9475

Swiss Prot: O75116

Source: KLH conjugated synthetic peptide derived from human ROCK2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq, Jul 2008].

Size: 100ul

Concentration: 1mg/ml

Applications: WB=1:500-1000

Predicted Molecular Weight: 161


Cross Reactive Species: Human

Predicted Cross Reactive Species: Mouse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:1000 Primary ab incubation condition: 4°C overnight Lysate: 1: HeLa, 2: MCF-7, 3: NIH/3T3 Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 161 kDa Observed MW: 161 kDa


Lane 1: Human HepG2 cell lysates; Lane 2: Human Hela cell lysates probed with ROCK2 Monoclonal Antibody, Unconjugated (bsm-60013M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.