bsm-60007M [Primary Antibody]
Mitofusin 2 monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: Mitofusin 2

Clonality: Monoclonal

Isotype: IgG2a

Entrez Gene: 9927

Swiss Prot: O95140

Source: KLH conjugated synthetic peptide derived from human Mitofusin 2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

This gene encodes a mitochondrial membrane protein that participates in mitochondrial fusion and contributes to the maintenance and operation of the mitochondrial network. This protein is involved in the regulation of vascular smooth muscle cell proliferation, and it may play a role in the pathophysiology of obesity. Mutations in this gene cause Charcot-Marie-Tooth disease type 2A2, and hereditary motor and sensory neuropathy VI, which are both disorders of the peripheral nervous system. Defects in this gene have also been associated with early-onset stroke. Two transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008].

Size: 100ul

Concentration: 1mg/ml

Applications: WB=1:1000

Predicted Molecular Weight: 83


Cross Reactive Species: Human

Predicted Cross Reactive Species: Mouse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Miao Song. et al. Mitophagy alleviates AIF-mediated spleen apoptosis induced by AlCl3 through Parkin stabilization in mice. FOOD CHEM TOXICOL. 2023 Jun;176:113762Read more>>
VALIDATION IMAGES

Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:1000 Primary ab incubation condition: 2 hours at room temperature Lysate: HepG2, Neuro-2a, EL4.IL-2 Protein loading quantity: 20 μg Exposure time: 60 S Predicted MW: 86 kDa Observed MW: 86 kDa


Lane 1: Mouse NIH/3T3 cell lysates; Lane 2: Human HeLa cell lysates; Lane 3: Human HepG2 cell lysates probed with Mitofusin 2 Monoclonal Antibody, Unconjugated (bsm-60007M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.