Host: Rabbit
Target Protein: IL18
Immunogen Range: 1-192/192
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 3606
Swiss Prot: Q14116
Source: Recombinant human IL18 protein
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
IL18 (IFN-gamma-inducing factor, IGIF) is a novel pro-inflammatory cytokine that augments natural killer (NK) activity in spleen cells. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity.
Size: 100ul
Concentration: 1ug/ul
Predicted Molecular Weight: 22
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
Lane 1: Normal HeLa cell lysates; Lane 2: HeLa cell treated with Brefeldin A (5 μ g/ml, 15 min) lysates;Lane 3: Jurkat cell lysates (Negative control ) probed with IL18 Monoclonal Antibody, Unconjugated (bsm-56205R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.
Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium EDTA buffer (Ph9.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (IL18) Monoclonal Antibody, Unconjugated (bsm-56205R) at 1:250 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium EDTA buffer (Ph9.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (IL18) Monoclonal Antibody, Unconjugated (bsm-56205R) at 1:250 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.