bsm-54767R [Primary Antibody]
CD44 Recombinant Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CD44 Recombinant

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 960

Swiss Prot: P16070

Source: KLH conjugated synthetic peptide derived from human CD44

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Size: 100ul

Concentration: 1mg/ml

Applications: WB(1:300-5000)

Predicted Molecular Weight: 85


Cross Reactive Species: Human

Predicted Cross Reactive Species: Mouse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Human A549 cell lysates; Lane 2: Human HeLa cell lysates; Lane 3: Human HUVEC cell lysates; Lane 4: Human U251 cell lysates probed with CD44 Monoclonal Antibody, Unconjugated (bsm-54767R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


25 ug total protein per lane of various lysates (see on figure) probed with CD44 monoclonal antibody, unconjugated (bsm-54767R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.