bsm-54573R [Primary Antibody]
Phospho-YAP1 (S127) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Phospho-YAP1 (S127)

Modification Site: Ser127

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 10413

Swiss Prot: P46937

Source: Human breast carcinoma tissue, human kidney tissue, mouse kidney tissue.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

This gene encodes the human ortholog of chicken YAP protein which binds to the SH3 domain of the Yes proto-oncogene product. This protein contains a WW domain that is found in various structural, regulatory and signaling molecules in yeast, nematode, and mammals, and may be involved in protein-protein interaction. [provided by RefSeq].

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 65


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Shao Zifei. et al. Exosomes derived from adipose tissues accelerate fibroblasts and keratinocytes proliferation and cutaneous wound healing via miR-92a/Hippo-YAP axis. J PHYSIOL BIOCHEM. 2023 Dec;:1-16Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Phospho-YAP1 (S127) Monoclonal Antibody, Unconjugated (bsm-54573R) at 1:50 for 30 minutes at room temperature, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Phospho-YAP1 (S127) Monoclonal Antibody, Unconjugated (bsm-54573R) at 1:50 for 30 minutes at room temperature, DAB staining.