Host: Rabbit
Target Protein: FASN
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 2194
Swiss Prot: P49327
Source: Recombinant human Fatty Acid Synthase
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1mg/ml
Applications:
ELISA(1:500-1000)
IHC-P(1:200-400)
Predicted Molecular Weight: 273
Cross Reactive Species: Human
Predicted Cross Reactive Species:
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
Paraformaldehyde-fixed, paraffin embedded (human breast); Antigen retrieval by microwaving in EDTA buffer (Ph8.0) for 5min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Fatty Acid Synthase) Monoclonal Antibody, Unconjugated (bsm-54469R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by microwaving in EDTA buffer (Ph8.0) for 5min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Fatty Acid Synthase) Monoclonal Antibody, Unconjugated (bsm-54469R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.