bsm-54460R [Primary Antibody]
NDUFS4 (2F3) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: NDUFS4

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 4724

Swiss Prot: O43181

Source: Full length recombinant protein.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 20


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Rat Heart; Probed with NDUFS4 (2F3) Monoclonal Antibody (bsm-54460R) at 1:500, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Paraformaldehyde-fixed and paraffin-embedded Rat Uterus tissue incubated with NDUFS4 (2F3) Monoclonal Antibody (bsm-54460R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.


Paraformaldehyde-fixed and paraffin-embedded Human Breast Cancer tissue incubated with NDUFS4 (2F3) Monoclonal Antibody (bsm-54460R) at 1:100, 30 min at room temperature, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.


Paraformaldehyde-fixed and paraffin-embedded Human Small Intestine tissue incubated with NDUFS4 (2F3) Monoclonal Antibody (bsm-54460R) at 1:100, 30 min at room temperature, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.


Paraformaldehyde-fixed and paraffin-embedded Mouse Kidney tissue incubated with NDUFS4 (2F3) Monoclonal Antibody (bsm-54460R) at 1:100, 30 min at room temperature, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.


Flow cytometric analysis of 293T cells with NDUFS4 (2F3) Monoclonal Antibody (bsm-54460R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).