bsm-54458R [Primary Antibody]
CD79a (1B12) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CD79a

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 973

Swiss Prot: P11912

Source: Recombinant protein within human CD79a aa 33-143.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells. Stimulates SYK autophosphorylation and activation. Binds to BLNK, bringing BLNK into proximity with SYK and allowing SYK to phosphorylate BLNK. Also interacts with and increases activity of some Src-family tyrosine kinases. Represses BCR signaling during development of immature B-cells.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: Observed: 38kDa, Predicted: 28kDa


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Flow cytometric analysis of Daudi cells with CD79a (1B12) Monoclonal Antibody (bsm-54458R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).


Paraformaldehyde-fixed and paraffin-embedded Human Spleen tissue incubated with CD79a (1B12) Monoclonal Antibody (bsm-54458R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.


Paraformaldehyde-fixed and paraffin-embedded Human Appendix tissue incubated with CD79a (1B12) Monoclonal Antibody (bsm-54458R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.


Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with CD79a (1B12) Monoclonal Antibody (bsm-54458R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.


Lane 1: Daudi Cells; Lane 2: Raji Cells; Probed with CD79a (1B12) Monoclonal Antibody (bsm-54458R) at 1:500, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.