VALIDATION IMAGES
Lane 1: Daudi Cells; Lane 2: HepG2 Cells; Probed with CLASP1 (2B11) Monoclonal Antibody (bsm-54456R) at 1:500, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with CLASP1 (2B11) Monoclonal Antibody (bsm-54456R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with CLASP1 (2B11) Monoclonal Antibody (bsm-54456R) at 1:100 in SiHa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Rat Kidney tissue incubated with CLASP1 (2B11) Monoclonal Antibody (bsm-54456R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.
Paraformaldehyde-fixed and paraffin-embedded Human Appendix tissue incubated with CLASP1 (2B11) Monoclonal Antibody (bsm-54456R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.
Paraformaldehyde-fixed and paraffin-embedded Human Small Intestine tissue incubated with CLASP1 (2B11) Monoclonal Antibody (bsm-54456R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.
Paraformaldehyde-fixed and paraffin-embedded Mouse Colon tissue incubated with CLASP1 (2B11) Monoclonal Antibody (bsm-54456R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.
Flow cytometric analysis of SiHa cells with CLASP1 (2B11) Monoclonal Antibody (bsm-54456R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).