bsm-54456R [Primary Antibody]
CLASP1 (2B11) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CLASP1

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 23332

Swiss Prot: Q7Z460

Source: Recombinant protein within human CLASP1 aa 1-200.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 169


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Daudi Cells; Lane 2: HepG2 Cells; Probed with CLASP1 (2B11) Monoclonal Antibody (bsm-54456R) at 1:500, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with CLASP1 (2B11) Monoclonal Antibody (bsm-54456R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with CLASP1 (2B11) Monoclonal Antibody (bsm-54456R) at 1:100 in SiHa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Rat Kidney tissue incubated with CLASP1 (2B11) Monoclonal Antibody (bsm-54456R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.


Paraformaldehyde-fixed and paraffin-embedded Human Appendix tissue incubated with CLASP1 (2B11) Monoclonal Antibody (bsm-54456R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.


Paraformaldehyde-fixed and paraffin-embedded Human Small Intestine tissue incubated with CLASP1 (2B11) Monoclonal Antibody (bsm-54456R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.


Paraformaldehyde-fixed and paraffin-embedded Mouse Colon tissue incubated with CLASP1 (2B11) Monoclonal Antibody (bsm-54456R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.


Flow cytometric analysis of SiHa cells with CLASP1 (2B11) Monoclonal Antibody (bsm-54456R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).