bsm-54451R [Primary Antibody]
Fbx32 Recombinant Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Fbx32

Immunogen Range: 1-200/355


Clonality: Recombinant

Isotype: IgG

Entrez Gene: 114907

Swiss Prot: Q969P5

Source: Recombinant human Fbx32

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Store at -20°C for 12 months.

Background:

Substrate recognition component of a (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 42


Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Shuang-Yan Chang. et al. miR-320 regulates myogenesis by targeting growth factor receptor-bound protein-2 and ameliorates myotubes atrophy. INT J BIOCHEM CELL B. 2022 Jun;147:106212Read more>>
  • Zhang Lijiao. et al. Dysregulated myokines and signaling pathways in skeletal muscle dysfunction in a cigarette smokeinduced model of chronic obstructive pulmonary disease. FRONT PHYSIOL. 2022 Aug;0:16Read more>>
  • Lijiao Zhang. et al. Myostatin/HIF2α-Mediated Ferroptosis is Involved in Skeletal Muscle Dysfunction in Chronic Obstructive Pulmonary Disease. INT J CHRONIC OBSTR. 2022 Sep;17:2383-2399Read more>>
  • Xuzhu Lin. et al. Undercarboxylated osteocalcin and ibandronate combination ameliorates hindlimb immobilization-induced muscle wasting. J PHYSIOL-LONDON. 2023 ApRead more>>
  • Zhao-Bo Luo. et al. Fecal transplant from myostatin deletion pigs positively impacts the gut-muscle axis. ELIFE. 2023; 12: e81858Read more>>
VALIDATION IMAGES

IF(ICC) staining with FBXO32 (2A3) Monoclonal Antibody (bsm-54451R) at 1:100 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with FBXO32 (2A3) Monoclonal Antibody (bsm-54451R) at 1:100 in L6 Cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


25 ug total protein per lane of various lysates (see on figure) probed with Fbx32 monoclonal antibody, unconjugated (bsm-54451R) at 1:10000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.