bsm-54446R [Primary Antibody]
Syntaxin 3 (2B1) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: STX3

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 6809

Swiss Prot: Q13277

Source: Recombinant protein corresponding to cytoplasmic region of human Syntaxin 3.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Syntaxin-3 is targeted to the apical membrane of epithelial cells where it forms clusters and is important in establishing and maintaining polarity necessary for protein trafficking involving vesicle fusion and exocytosis. It is involved in docking of synaptic vesicles at presynaptic active zones.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 33


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: SH-SY5Y Cells; Lane 2: Human Kidney; Probed with Syntaxin 3 (2B1) Monoclonal Antibody (bsm-54446R) at 1:1000, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Paraformaldehyde-fixed and paraffin-embedded Rat Kidney tissue incubated with Syntaxin 3 (2B1) Monoclonal Antibody (bsm-54446R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Prostate Cancer tissue incubated with Syntaxin 3 (2B1) Monoclonal Antibody (bsm-54446R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with Syntaxin 3 (2B1) Monoclonal Antibody (bsm-54446R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse Pancreas tissue incubated with Syntaxin 3 (2B1) Monoclonal Antibody (bsm-54446R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Flow cytometric analysis of 293T cells with Syntaxin 3 (2B1) Monoclonal Antibody (bsm-54446R) at a 1:100 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).