bsm-54444R [Primary Antibody]
FIS1 (4A8) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: FIS1

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 51024

Swiss Prot: Q9Y3D6

Source: Synthetic peptide corresponding to N-terminal Human TTC11.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Involved in the fragmentation of the mitochondrial network and its perinuclear clustering. Plays a minor role in the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface and mitochondrial fission. Can induce cytochrome c release from the mitochondrion to the cytosol, ultimately leading to apoptosis. Also mediates peroxisomal fission.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 17


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: SK-Br-3 Cells; Probed with FIS1 (4A8) Monoclonal Antibody (bsm-54444R) at 1:2000, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with FIS1 (4A8) Monoclonal Antibody (bsm-54444R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with FIS1 (4A8) Monoclonal Antibody (bsm-54444R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with FIS1 (4A8) Monoclonal Antibody (bsm-54444R) at 1:100 in SK-Br-3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with FIS1 (4A8) Monoclonal Antibody (bsm-54444R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Small Intestine tissue incubated with FIS1 (4A8) Monoclonal Antibody (bsm-54444R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Uterus tissue incubated with FIS1 (4A8) Monoclonal Antibody (bsm-54444R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.