bsm-54435R
[Primary Antibody]
BANF1 (11H3) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: BANF1
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 8815
Swiss Prot: O75531
Source: Full length recombinant protein of human BANF1.
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Barrier-to-autointegration factor (BANF1) binds non-specifically to double stranded DNA, possibly to play a role in tissue- or cell type-specific gene expression by interacting with different homeodomain transcription factors. BANF1 compresses chromatin structure and interacts with the LEM domain of nuclear proteins to play a crucial role in membrane recruitment and chromatin decondensation during nuclear assembly. Additionally, retroviruses like HIV-1 incorporate BANF1 from host cells into preintegration complexes (PICs) to prevent autointegration of retroviral DNA and thereby promote integration of retroviral DNA into the host chromosome.
Size: 100ul
Concentration: 1ug/ul
Predicted Molecular Weight: 10
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
IF(ICC) staining with BANF1 (11H3) Monoclonal Antibody (bsm-54435R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human Thyroid tissue incubated with BANF1 (11H3) Monoclonal Antibody (bsm-54435R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with BANF1 (11H3) Monoclonal Antibody (bsm-54435R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Heart tissue incubated with BANF1 (11H3) Monoclonal Antibody (bsm-54435R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Rat Epididymis tissue incubated with BANF1 (11H3) Monoclonal Antibody (bsm-54435R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse Testis tissue incubated with BANF1 (11H3) Monoclonal Antibody (bsm-54435R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Flow cytometric analysis of MCF-7 cells with BANF1 (11H3) Monoclonal Antibody (bsm-54435R) at a 1:100 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).
Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with BANF1 (11H3) Monoclonal Antibody, Unconjugated (bsm-54435R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human esophageal carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with BANF1 (11H3) Monoclonal Antibody, Unconjugated (bsm-54435R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with BANF1 (11H3) Monoclonal Antibody, Unconjugated (bsm-54435R) at 1:200 overnight at 4°C, DAB staining.