bsm-54434R [Primary Antibody]
BRD2 Recombinant Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: BRD2

Immunogen Range: 1-50/801


Clonality: Recombinant

Isotype: IgG

Entrez Gene: 6046

Swiss Prot: P25440

Source: Recombinant protein within human BRD2 aa 1-200.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

The bromodomain-containing proteins include BRD2, BRD3, BRD4 and BRDT. BRD2 (RING3 protein) is a mitogen-activated nuclear protein whose gene is located in the human MHC II region, suggesting its relation to HLA-associated diseases. The bromodomain-containing proteins are ubiquitously expressed. BRD2 may play a role in spermatogenesis or folliculogenesis (By similarity). BRD2 binds hyperacetylated chromatin and plays a role in the regulation of transcription, probably by chromatin remodeling. Regulates transcription of the CCND1 gene. Plays a role in nucleosome assembly.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 88


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: SiHa Cells; Probed with BRD2 (6B5) Monoclonal Antibody (bsm-54434R) at 1:2000, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with BRD2 (6B5) Monoclonal Antibody (bsm-54434R) at 1:100 in 293T cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with BRD2 (6B5) Monoclonal Antibody (bsm-54434R) at 1:100 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with BRD2 (6B5) Monoclonal Antibody (bsm-54434R) at 1:100 in SiHa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BRD2 Monoclonal Antibody, Unconjugated(bsm-54434R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Flow cytometric analysis of 293T cells with BRD2 (6B5) Monoclonal Antibody (bsm-54434R) at a 1:100 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).