bsm-54428R [Primary Antibody]
O-GlcNAcase (1A5) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: OGA

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 10724

Swiss Prot: O60502

Source: Recombinant protein of N-terminal human MGEA5.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Isoform 1: - Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro). Does not bind acetyl-CoA and does not have histone acetyltransferase activity. Isoform 3: - Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro), but has about six times lower specific activity than isoform 1.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 103


Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed and paraffin-embedded Mouse Testis tissue incubated with O-GlcNAcase (1A5) Monoclonal Antibody (bsm-54428R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Pancreas tissue incubated with O-GlcNAcase (1A5) Monoclonal Antibody (bsm-54428R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with O-GlcNAcase (1A5) Monoclonal Antibody (bsm-54428R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Lane 1: PC-3M Cells; Lane 2: SH-SY5Y Cells; Probed with O-GlcNAcase (1A5) Monoclonal Antibody (bsm-54428R) at 1:500, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.