bsm-54425R [Primary Antibody]
Methionine Aminopeptidase 2 (2G3) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: METAP2

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 10988

Swiss Prot: P50579

Source: Recombinant protein within C-terminal human Methionine Aminopeptidase 2.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Methionine aminopeptidases (MetAP), also designated peptidase M proteins, are members of the M24 family of proteins. Both MetAP-1 and MetAP-2 release N-terminal amino acids, usually methionine, from nascent peptides and arylamines. Eukaryotes contain both MetAP-1 and MetAP-2, whereas prokaryotes possess only the MetAP-1 enzyme. MetAP-1 and MetAP-2 control cell proliferation in mammalian cells. MetAP-2 is highly conserved between human and Saccharomyces cerevisiae. Neurofibromin (NF1) regulates MetAp-2 and increased expression of MetAP-2 correlates with several forms of cancer. Inhibitors of MetAP-2 are potential targets in cancer therapeutics, particularly in NF1-associated tumor proliferation. Chemotherapeutic drugs such as ovalicin and fumagillin bind to the active site of and inhibit MetAp-2.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 53


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Daudi Cells; Lane 2: K562 Cells; Lane 3: Mouse Thymus; Lane 4: Mouse Kidney; Probed with Methionine Aminopeptidase 2 (2G3) Monoclonal Antibody (bsm-54425R) at 1:1000, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with Methionine Aminopeptidase 2 (2G3) Monoclonal Antibody (bsm-54425R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with Methionine Aminopeptidase 2 (2G3) Monoclonal Antibody (bsm-54425R) at 1:100 in PC-3M cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with Methionine Aminopeptidase 2 (2G3) Monoclonal Antibody (bsm-54425R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Mouse Seminal Vesicle tissue incubated with Methionine Aminopeptidase 2 (2G3) Monoclonal Antibody (bsm-54425R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with Methionine Aminopeptidase 2 (2G3) Monoclonal Antibody (bsm-54425R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Prostate Cancer tissue incubated with Methionine Aminopeptidase 2 (2G3) Monoclonal Antibody (bsm-54425R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with Methionine Aminopeptidase 2 (2G3) Monoclonal Antibody (bsm-54425R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse Testis tissue incubated with Methionine Aminopeptidase 2 (2G3) Monoclonal Antibody (bsm-54425R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Flow cytometric analysis of Daudi cells with Methionine Aminopeptidase 2 (2G3) Monoclonal Antibody (bsm-54425R) at a 1:100 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).