bsm-54422R [Primary Antibody]
FKBP4 (12A5) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: FKBP4

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 2288

Swiss Prot: Q02790

Source: Recombinant protein within human FKBP52 aa 1-200.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Immunophilin protein with PPIase and co-chaperone activities. Component of steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). May play a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors between cytoplasm and nuclear compartments. The isomerase activity controls neuronal growth cones via regulation of TRPC1 channel opening. Acts also as a regulator of microtubule dynamics by inhibiting MAPT/TAU ability to promote microtubule assembly. May have a protective role against oxidative stress in mitochondria.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 52


Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: 293 Cells; Lane 2: SK-Br-3 Cells; Probed with FKBP4 (12A5) Monoclonal Antibody (bsm-54422R) at 1:2000, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with FKBP4 (12A5) Monoclonal Antibody (bsm-54422R) 1:100 in 293T cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with FKBP4 (12A5) Monoclonal Antibody (bsm-54422R) 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Human Prostate Cancer tissue incubated with FKBP4 (12A5) Monoclonal Antibody (bsm-54422R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Esophagus tissue incubated with FKBP4 (12A5) Monoclonal Antibody (bsm-54422R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse Brain tissue incubated with FKBP4 (12A5) Monoclonal Antibody (bsm-54422R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Flow cytometric analysis of 293T cells with FKBP4 (12A5) Monoclonal Antibody (bsm-54422R) at a 1:100 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).