bsm-54411R [Primary Antibody]
TNPO3 (11F2) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: TNPO3

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 23534

Swiss Prot: Q9Y5L0

Source: Recombinant protein within human TNPO3 aa 800-950.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

TNPO3 is a nuclear import receptor for serine/arginine-rich (SR) proteins such as the splicing factors SFRS1 and SFRS2 by recognizing phosphorylated RS domains. TNPO3 has also been shown to be involved in HIV-1 infection, apparently through interaction with the HIV-1 capsid protein. Two transcript variants encoding different isoforms as well as a noncoding transcript have been found for this gene

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 104


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: K562 Cells; Lane 2: HL-60 Cells; Lane 3: Mouse Placenta; Probed with TNPO3 (11F2) Monoclonal Antibody (bsm-54411R) at 1:1000, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with TNPO3 (11F2) Monoclonal Antibody (bsm-54411R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with TNPO3 (11F2) Monoclonal Antibody (bsm-54411R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with TNPO3 (11F2) Monoclonal Antibody (bsm-54411R) at 1:100 in SiHa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Human Liver Cancer tissue incubated with TNPO3 (11F2) Monoclonal Antibody (bsm-54411R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Small Intestine tissue incubated with TNPO3 (11F2) Monoclonal Antibody (bsm-54411R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse Lung tissue incubated with TNPO3 (11F2) Monoclonal Antibody (bsm-54411R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse Kidney tissue incubated with TNPO3 (11F2) Monoclonal Antibody (bsm-54411R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.