DATASHEET
Host:
Rabbit
Target Protein:
TYROBP
Clonality:
Monoclonal
Isotype:
IgG
Entrez Gene:
7305
Swiss Prot:
O43914
Source:
Synthetic peptide within human DAP12 aa 60-100.
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at -20°C for 12 months.
Background:
TYROBP is a transmembrane signaling polypeptide which contains an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. The encoded protein may associate with the killer-cell inhibitory receptor (KIR) family of membrane glycoproteins and may act as an activating signal transduction element. This protein may bind zeta-chain (TCR) associated protein kinase 70kDa (ZAP-70) and spleen tyrosine kinase (SYK) and play a role in signal transduction, bone modeling, brain myelination, and inflammation. Mutations within this gene have been associated with polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL), also known as Nasu-Hakola disease. Its putative receptor, triggering receptor expressed on myeloid cells 2 (TREM2), also causes PLOSL. Multiple alternative transcript variants encoding distinct isoforms have been identified for this gene. TYROBP Non-covalently associates with activating receptors of the CD300 family. Cross-linking of CD300-TYROBP complexes results in cellular activation. Involved for instance in neutrophil activation mediated by integrin.
VALIDATION IMAGES
Lane 1: THP-1 Cells; Probed with TYROBP (2F4 ) Monoclonal Antibody (bsm-54408R) at 1:500, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed and paraffin-embedded Human Lung Cancer tissue incubated with TYROBP (2F4 ) Monoclonal Antibody (bsm-54408R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Flow cytometric analysis of K562 cells with TYROBP (2F4 ) Monoclonal Antibody (bsm-54408R) at a 1:100 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).