bsm-54404R [Primary Antibody]
NCF2 (3A1) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: NCF2

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 4688

Swiss Prot: P19878

Source: Recombinant protein within Human NCF2 aa 150-300.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Neutrophil cytosolic factor 2, is the 67-kilodalton cytosolic subunit of the multi-protein NADPH oxidase complex found in neutrophils. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in this gene, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease, a disease that causes recurrent infections by catalase-positive organisms. Alternative splicing results in multiple transcript variants encoding different isoforms. NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 60/47


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed and paraffin-embedded Rat Spleen tissue incubated with NCF2 (3A1) Monoclonal Antibody (bsm-54404R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Spleen tissue incubated with NCF2 (3A1) Monoclonal Antibody (bsm-54404R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse Spleen tissue incubated with NCF2 (3A1) Monoclonal Antibody (bsm-54404R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Lane 1: Rat Spleen; Probed with NCF2 (3A1) Monoclonal Antibody (bsm-54404R) at 1:500, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.