VALIDATION IMAGES
Lane 1: Rat Liver; Probed with ADH1A (2G4) Monoclonal Antibody (bsm-54396R) at 1:1000, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with ADH1A (2G4) Monoclonal Antibody (bsm-54396R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human Liver tissue incubated with ADH1A (2G4) Monoclonal Antibody (bsm-54396R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Rat Liver tissue incubated with ADH1A (2G4) Monoclonal Antibody (bsm-54396R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse Liver tissue incubated with ADH1A (2G4) Monoclonal Antibody (bsm-54396R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Lane 1: Rat Liver tissue lysates probed with ADH1A Monoclonal Antibody, Unconjugated (bsm-54396R) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.