bsm-54381R [Primary Antibody]
Cullin 2 (4C9) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CUL2

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 8453

Swiss Prot: Q13617

Source: Recombinant protein within human Cullin 2 aa 1-200.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. ECS complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins (PubMed:27565346). May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF).

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 89


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with Cullin 2 (4C9) Monoclonal Antibody (bsm-54381R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Rat Testis tissue incubated with Cullin 2 (4C9) Monoclonal Antibody (bsm-54381R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining.


Lane 1: PC-3M Cells; Lane 2: K562 Cells; Probed with Cullin 2 (4C9) Monoclonal Antibody (bsm-54381R) at 1:1000, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.