bsm-54380R [Primary Antibody]
Fibrinogen gamma chain (10A1) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: FGG

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 2266

Swiss Prot: P02679

Source: Recombinant protein corresponding to human Fibrinogen gamma50-250aa.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

Together with fibrinogen alpha (FGA) and fibrinogen beta (FGB), polymerizes to form an insoluble fibrin matrix. Has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets via an ITGB3-dependent pathway. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the antibacterial immune response via both innate and T-cell mediated pathways.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 52


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Human Plasma; Probed with Fibrinogen gamma chain (10A1) Monoclonal Antibody (bsm-54380R) at 1:5000, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Paraformaldehyde-fixed and paraffin-embedded Human colon tissue incubated with Fibrinogen gamma chain (10A1) Monoclonal Antibody (bsm-54380R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Flow cytometric analysis of Hela cells with Fibrinogen gamma chain (10A1) Monoclonal Antibody (bsm-54380R) at a 1:50 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).