bsm-54364R [Primary Antibody]
IRAK2 (7A4) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: IRAK2

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 3656

Swiss Prot: O43187

Source: Recombinant protein within human IRAK2 aa 1-200.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

IRAK2 encodes the interleukin-1 receptor-associated kinase 2, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. IRAK2 is reported to participate in the IL1-induced upregulation of NF-kappaB. IRAK2 has been shown to interact with TRAF6 and Myd88. Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 69


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Flow cytometric analysis of Jurkat cells with IRAK2 (7A4) Monoclonal Antibody (bsm-54364R) at a 1:100 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).


Paraformaldehyde-fixed and paraffin-embedded Human Heart tissue incubated with IRAK2 (7A4) Monoclonal Antibody (bsm-54364R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Prostate tissue incubated with IRAK2 (7A4) Monoclonal Antibody (bsm-54364R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with IRAK2 (7A4) Monoclonal Antibody (bsm-54364R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


IF(ICC) staining with IRAK2 (7A4) Monoclonal Antibody (bsm-54364R) at 1:100 in PC-3M cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Lane 1: PC-3M Cells; Lane 2: A549 Cells; Lane 3: HepG2 Cells; Probed with IRAK2 (7A4) Monoclonal Antibody (bsm-54364R) at 1:500, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.