bsm-54363R [Primary Antibody]
UQCRFS1 (10C1) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: UQCRFS1

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 7386

Swiss Prot: P47985

Source: Recombinant protein within human UQCRFS1 aa 100 to the C-terminus.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

Cytochrome b-c1 complex subunit Rieske, mitochondrial: Component of the mitochondrial ubiquinol-cytochrome c reductase complex dimer (complex III dimer), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis. Incorporation of UQCRFS1 is the penultimate step in complex III assembly.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 21


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Flow cytometric analysis of A549 cells with UQCRFS1 (10C1) Monoclonal Antibody (bsm-54363R) at a 1:100 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).


Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with UQCRFS1 (10C1) Monoclonal Antibody (bsm-54363R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Appendix tissue incubated with UQCRFS1 (10C1) Monoclonal Antibody (bsm-54363R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse Cerebellum tissue incubated with UQCRFS1 (10C1) Monoclonal Antibody (bsm-54363R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with UQCRFS1 (10C1) Monoclonal Antibody (bsm-54363R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Rat Skeletal Muscle tissue incubated with UQCRFS1 (10C1) Monoclonal Antibody (bsm-54363R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


IF(ICC) staining with UQCRFS1 (10C1) Monoclonal Antibody (bsm-54363R) at 1:100 in SiHa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with UQCRFS1 (10C1) Monoclonal Antibody (bsm-54363R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with UQCRFS1 (10C1) Monoclonal Antibody (bsm-54363R) at 1:100 in 293T cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Lane 1: A549 Cells; Lane 2: 293 Cells; Lane 3: Mouse Kidney; Probed with UQCRFS1 (10C1) Monoclonal Antibody (bsm-54363R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


25 ug total protein per lane of various lysates (see on figure) probed with UQCRFS1 monoclonal antibody, unconjugated (bsm-54363R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.