bsm-54362R [Primary Antibody]
SULT2A1 (3A1) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: SULT2A1

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 6822

Swiss Prot: Q06520

Source: Recombinant protein within human SULT2A1 aa 100 to the C-terminus.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfonation of steroids and bile acids in the liver and adrenal glands.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 34


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Mouse Kidney; Probed with SULT2A1 (3A1) Monoclonal Antibody (bsm-54362R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with SULT2A1 (3A1) Monoclonal Antibody (bsm-54362R) at 1:100 in 293T cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with SULT2A1 (3A1) Monoclonal Antibody (bsm-54362R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Rat Adrenal Gland tissue incubated with SULT2A1 (3A1) Monoclonal Antibody (bsm-54362R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Liver tissue incubated with SULT2A1 (3A1) Monoclonal Antibody (bsm-54362R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Liver Cancer tissue incubated with SULT2A1 (3A1) Monoclonal Antibody (bsm-54362R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Small Intestine tissue incubated with SULT2A1 (3A1) Monoclonal Antibody (bsm-54362R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse Liver tissue incubated with SULT2A1 (3A1) Monoclonal Antibody (bsm-54362R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Flow cytometric analysis of HepG2 cells with SULT2A1 (3A1) Monoclonal Antibody (bsm-54362R) at a 1:100 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).