bsm-54350R [Primary Antibody]
CHD1L (8A1) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CHD1L

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 9557

Swiss Prot: Q86WJ1

Source: Recombinant human CHD1L between 600 to 800 amino acids.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

DNA helicase which plays a role in chromatin-remodeling following DNA damage. Targeted to sites of DNA damage through interaction with poly(ADP-ribose) and functions to regulate chromatin during DNA repair. Able to catalyze nucleosome sliding in an ATP-dependent manner. Helicase activity is strongly stimulated upon poly(ADP-ribose)-binding.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 101/90


Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: HepG2 Cells; Probed with CHD1L (8A1) Monoclonal Antibody (bsm-54350R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with CHD1L (8A1) Monoclonal Antibody (bsm-54350R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.


Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with CHD1L (8A1) Monoclonal Antibody (bsm-54350R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.


Paraformaldehyde-fixed and paraffin-embedded Mouse Testis tissue incubated with CHD1L (8A1) Monoclonal Antibody (bsm-54350R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.


Flow cytometric analysis of HepG2 cells with CHD1L (8A1) Monoclonal Antibody (bsm-54350R) at a 1:100 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).