VALIDATION IMAGES
Lane 1: HepG2 Cells; Probed with CHD1L (8A1) Monoclonal Antibody (bsm-54350R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with CHD1L (8A1) Monoclonal Antibody (bsm-54350R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.
Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with CHD1L (8A1) Monoclonal Antibody (bsm-54350R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.
Paraformaldehyde-fixed and paraffin-embedded Mouse Testis tissue incubated with CHD1L (8A1) Monoclonal Antibody (bsm-54350R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.
Flow cytometric analysis of HepG2 cells with CHD1L (8A1) Monoclonal Antibody (bsm-54350R) at a 1:100 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).