bsm-54346R [Primary Antibody]
Nuf2 (4A3) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Nuf2

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 83540

Swiss Prot: Q9BZD4

Source: Recombinant protein within human Nuf2 aa 1-200.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 54


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: K562 Cells; Lane 2: PC-3M Cells; Lane 3: THP-1 Cells; Probed with Nuf2 (4A3) Monoclonal Antibody (bsm-54346R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Flow cytometric analysis of A431 cells with Nuf2 (4A3) Monoclonal Antibody (bsm-54346R) at a 1:50 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).