bsm-54344R [Primary Antibody]
PARP2 (19A1) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: PARP2

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 10038

Swiss Prot: Q9UGN5

Source: Recombinant protein within the C-terminus of human PARP2.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

Poly-ADP-ribosyltransferase that mediates poly-ADP-ribosylation of proteins and plays a key role in DNA repair. Mainly mediates glutamate and aspartate ADP-ribosylation of target proteins: the ADP-D-ribosyl group of NAD+ is transferred to the acceptor carboxyl group of glutamate and aspartate residues and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units. ADP-ribosylation follows DNA damage and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Also mediates serine ADP-ribosylation of target proteins following interaction with HPF1; HPF1 conferring serine specificity. In addition to proteins, also able to ADP-ribosylate DNA: preferentially acts on 5'-terminal phosphates at DNA strand breaks termini in nicked duplex

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Flow cytometric analysis of SH-SY5Y cells with PARP2 (19A1) Monoclonal Antibody (bsm-54344R) at a 1:50 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).


Lane 1: SiHa Cells; Lane 2: Raji Cells; Probed with PARP2 (19A1) Monoclonal Antibody (bsm-54344R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.