bsm-54342R [Primary Antibody]
ERp29 (1A11) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ERp29

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 10961

Swiss Prot: P30040

Source: Recombinant protein within human ERp29 aa 1-150.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

Does not seem to be a disulfide isomerase. Plays an important role in the processing of secretory proteins within the endoplasmic reticulum (ER), possibly by participating in the folding of proteins in the ER.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 29


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: THP-1 Cells; Probed with ERp29 (1A11) Monoclonal Antibody (bsm-54342R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with ERp29 (1A11) Monoclonal Antibody (bsm-54342R) at 1:100 in F9 Cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with ERp29 (1A11) Monoclonal Antibody (bsm-54342R) at 1:100 in SiHa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with ERp29 (1A11) Monoclonal Antibody (bsm-54342R) at 1:100 in PMVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Rat Testis tissue incubated with ERp29 (1A11) Monoclonal Antibody (bsm-54342R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Thyroid tissue incubated with ERp29 (1A11) Monoclonal Antibody (bsm-54342R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with ERp29 (1A11) Monoclonal Antibody (bsm-54342R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.