bsm-54340R [Primary Antibody]
Glucose 6 phosphate isomerase (3A1) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: GPI

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 2821

Swiss Prot: P06744

Source: Recombinant protein corresponding to human Glucose 6 phosphate isomerase 300-500aa.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

Glucose-6-phosphate isomerase (GPI) has many other names, including Phosphohexose isomerase (PHI), Neuroleukin (NLK) and Spermantigen-36 (SA-36). GPI is a cytoplasmic homodimer belonging to the GPI family. It is a neurotrophic factor for spinal and sensory neurons and is involved in glycolysis and gluconeogenesis. Defects or mutations in GPI can cause hereditary nonspherocytic hemolytic anemia (HA), hydrops fetalis, immediate neonatal death and neurological impairment.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 63


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: A549 Cells; Probed with Glucose 6 phosphate isomerase (3A1) Monoclonal Antibody (bsm-54340R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with Glucose 6 phosphate isomerase (3A1) Monoclonal Antibody (bsm-54340R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with Glucose 6 phosphate isomerase (3A1) Monoclonal Antibody (bsm-54340R) at 1:100 in LO2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Human Prostate tissue incubated with Glucose 6 phosphate isomerase (3A1) Monoclonal Antibody (bsm-54340R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with Glucose 6 phosphate isomerase (3A1) Monoclonal Antibody (bsm-54340R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Colon tissue incubated with Glucose 6 phosphate isomerase (3A1) Monoclonal Antibody (bsm-54340R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Flow cytometric analysis of A549 cells with Glucose 6 phosphate isomerase (3A1) Monoclonal Antibody (bsm-54340R) at a 1:100 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).