bsm-54334R [Primary Antibody]
CREB1 (Ser133) (5F2) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CREB1 (Ser133)

Modification Site: Ser133

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 1385

Swiss Prot: P16220

Source: Synthetic phospho-peptide corresponding to residues surrounding Ser133 of human Creb

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 37


Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Human Colon; Probed with CREB1 (Ser133) (5F2) Monoclonal Antibody (bsm-54334R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with CREB1 (Ser133) (5F2) Monoclonal Antibody (bsm-54334R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with CREB1 (Ser133) (5F2) Monoclonal Antibody (bsm-54334R) at 1:100 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Human Spleen tissue incubated with CREB1 (Ser133) (5F2) Monoclonal Antibody (bsm-54334R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse Colon tissue incubated with CREB1 (Ser133) (5F2) Monoclonal Antibody (bsm-54334R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with CREB1 (Ser133) (5F2) Monoclonal Antibody (bsm-54334R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Flow cytometric analysis of HUVEC cells with CREB1 (Ser133) (5F2) Monoclonal Antibody (bsm-54334R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).