bsm-54320R [Primary Antibody]
eEF1A1 (3F9) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: eEF1A1

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 1915

Swiss Prot: P68104

Source: Recombinant protein within C-terminal human eEF1A1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. With PARP1 and TXK, forms a complex that acts as a T helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production.

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Rat brain tissue; Lane 2: Mouse skeletal muscle; Lane 3: Mouse cerebellum; Lane 4: Rat skin; Lane 5: Daudi Cells; Probed with eEF1A1 (3F9) Monoclonal Antibody (bsm-54320R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with eEF1A1 (3F9) Monoclonal Antibody (bsm-54320R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with eEF1A1 (3F9) Monoclonal Antibody (bsm-54320R) at 1:100 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with eEF1A1 (3F9) Monoclonal Antibody (bsm-54320R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Rat Skeletal Muscle tissue incubated eEF1A1 (3F9) Monoclonal Antibody (bsm-54320R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated eEF1A1 (3F9) Monoclonal Antibody (bsm-54320R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Liver Cancer tissue incubated eEF1A1 (3F9) Monoclonal Antibody (bsm-54320R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated eEF1A1 (3F9) Monoclonal Antibody (bsm-54320R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse Smooth Muscle tissue incubated eEF1A1 (3F9) Monoclonal Antibody (bsm-54320R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Flow cytometric analysis of THP-1 cells with eEF1A1 (3F9) Monoclonal Antibody (bsm-54320R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).