VALIDATION IMAGES
Lane 1: Rat brain tissue; Lane 2: Mouse skeletal muscle; Lane 3: Mouse cerebellum; Lane 4: Rat skin; Lane 5: Daudi Cells; Probed with eEF1A1 (3F9) Monoclonal Antibody (bsm-54320R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with eEF1A1 (3F9) Monoclonal Antibody (bsm-54320R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with eEF1A1 (3F9) Monoclonal Antibody (bsm-54320R) at 1:100 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with eEF1A1 (3F9) Monoclonal Antibody (bsm-54320R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Rat Skeletal Muscle tissue incubated eEF1A1 (3F9) Monoclonal Antibody (bsm-54320R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated eEF1A1 (3F9) Monoclonal Antibody (bsm-54320R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Liver Cancer tissue incubated eEF1A1 (3F9) Monoclonal Antibody (bsm-54320R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated eEF1A1 (3F9) Monoclonal Antibody (bsm-54320R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse Smooth Muscle tissue incubated eEF1A1 (3F9) Monoclonal Antibody (bsm-54320R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Flow cytometric analysis of THP-1 cells with eEF1A1 (3F9) Monoclonal Antibody (bsm-54320R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).