bsm-54307R [Primary Antibody]
Glutathione Synthetase (2F9) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Glutathione Synthetase

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 2937

Swiss Prot: P48637

Source: Recombinant protein within human Glutathione Synthetase 1-200

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. Glutathione synthetase functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in this gene are a cause of glutathione synthetase deficiency.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 52


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: SiHa Cells; Lane 2: Mouse Colon; Lane 3: Rat Liver Probed with Glutathione Synthetase (2F9) Monoclonal Antibody (bsm-54307R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with Glutathione Synthetase (2F9) Monoclonal Antibody (bsm-54307R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with Glutathione Synthetase (2F9) Monoclonal Antibody (bsm-54307R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with Glutathione Synthetase (2F9) Monoclonal Antibody (bsm-54307R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with Glutathione Synthetase (2F9) Monoclonal Antibody (bsm-54307R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Rat Epididymis tissue incubated with Glutathione Synthetase (2F9) Monoclonal Antibody (bsm-54307R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embeddedMouse Colon tissue incubated with Glutathione Synthetase (2F9) Monoclonal Antibody (bsm-54307R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Flow cytometric analysis of HepG2 cells with Glutathione Synthetase (2F9) Monoclonal Antibody (bsm-54307R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).