bsm-54283R [Primary Antibody]
Furin Recombinant Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Furin

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 5045

Swiss Prot: P09958

Source: Recombinant protein within human Furin aa 200-400.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif. Mediates processing of TGFB1, an essential step in TGF-beta-1 activation. Probably cleaves and activates anthrax and diphtheria toxins. Required for H7N1 and H5N1 influenza virus infection probably by cleaving hemagglutinin.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:500-1:1000)
IHC-P(1:50-1:100)

Predicted Molecular Weight: 87


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Davaanyam Dashdulam. et al. HMGB1 induces hepcidin upregulation in astrocytes and causes an acute iron surge and subsequent ferroptosis in the postischemic brain. EXP MOL MED. 2023 Nov;:1-15Read more>>
VALIDATION IMAGES

Lane 1: HepG2 Cells; Lane 2: Hela Cells; Lane 3: Hela Cells; Lane 4: MCF-7 Cells; Probed with Furin (8C3) Monoclonal Antibody (bsm-54283R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Paraformaldehyde-fixed and paraffin-embedded Human Liver tissue incubated with Furin (8C3) Monoclonal Antibody (bsm-54283R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Colon tissue incubated with Furin (8C3) Monoclonal Antibody (bsm-54283R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Placenta tissue incubated with Furin (8C3) Monoclonal Antibody (bsm-54283R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse Brain tissue incubated with Furin (8C3) Monoclonal Antibody (bsm-54283R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.