bsm-54278R [Primary Antibody]
EPAS1 Recombinant Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: EPAS1

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 2034

Swiss Prot: Q99814

Source: Recombinant full length Human protein

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Transcription factor involved in the induction of oxygen regulated genes. Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation requires recruitment of transcriptional coactivators such as CREBBP and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 96


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed and paraffin-embedded Rat Lung tissue incubated with EPAS1 (2G3) Monoclonal Antibody (bsm-54278R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Breast Cancer tissue incubated with EPAS1 (2G3) Monoclonal Antibody (bsm-54278R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Placenta tissue incubated with EPAS1 (2G3) Monoclonal Antibody (bsm-54278R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse Colon tissue incubated with EPAS1 (2G3) Monoclonal Antibody (bsm-54278R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Flow cytometric analysis of HUVEC cells with EPAS1 (2G3) Monoclonal Antibody (bsm-54278R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).


Lane 1: Mouse Lung tissue lysates; Lane 2: Mouse Placenta tissue lysates; Lane 3: Mouse Testis tissue lysates; Lane 4: Rat Testis tissue lysates ; Lane 5: Human HepG2 cell lysates; Lane 6:Human MCF-7 cell lysates; Lane 7: Human U87MG cell lysates; Lane 8: Human SH-SY5Y cell lysates probed with EPAS1 (2G3) Monoclonal Antibody, Unconjugated (bsm-54278R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.